From Tilling

Example of mutation signal from sequencing data

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Rice gtoa.png

Figure 1. Detection of changes in a TILLED-by-sequencing rice gene. PCR products representing target genes were amplified from overlapping pools of genomic DNAs from mutagenized individuals. The y-axis represents the frequency of the given changes. The x-axis represents the base positions along one target gene. Mutations are identified by the triple occurrence of outliers.

For details see our 2011 paper in Plant Physiology and the data and methods page.

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